High Sensitivity Lc-ms/ms Method For Pthrp Analysis
ID U-5729
Category Diagnostics
Subcategory Biomarkers
Researchers
Brief Summary
A revolutionary LC-MS/MS method offering high sensitivity and specificity for measuring parathyroid hormone-related peptide (PTHrP) in biological samples
Problem Statement
Currently available methods for analysis of PTHrP, including Radioimmunoassay (RIA) and ELISA, have poor analytical performance, underestimate PTHrP concentration in patient samples, and have inadequate clinical sensitivity and specificity, leading to false negatives.
Technology Description
This technology introduces a method for measuring parathyroid hormone-related peptide (PTHrP) in biological samples, utilizing LC-MS/MS along with an isotope-labeled internal standard. By extracting PTHrP peptides, digesting them proteolytically, and analyzing specific digestion products, this method achieves unprecedented sensitivity and specificity in PTHrP quantification.
• Diagnostic testing for hypercalcemia and related conditions, improving the detection of PTHrP-secreting malignancies.
• Research tool for studying the role of PTHrP in calcium regulation, bone development, and other physiological functions.
• Potential use in drug development and therapeutic monitoring, particularly for treatments affecting calcium homeostasis.
Stage of Development
Clinical Validation
Benefit
- High sensitivity capable of detecting PTHrP concentrations as low as 0.5 pmol/L or less.
- High specificity through the use of isotope-labeled internal standards for accurate quantification.
- Rapid sample preparation process, completed in 2 hours or less to minimize PTHrP degradation.
- Utilization of affinity extraction for selective PTHrP peptide isolation, enhancing measurement precision.
- Capability to process without the need for reduction or alkylation reactions, simplifying the preparation steps.
Publications
Kushnir MM, Rockwood AL, Strathmann FG, Frank EL, Straseski JA, Meikle AW. LC-MS/MS Measurement of Parathyroid Hormone-Related Peptide. Clin Chem. 2016;62(1):218-226. doi:10.1373/clinchem.2015.244012
Kushnir MM, Rockwood AL. High Sensitivity Measurement of Parathyroid Hormone-Related Protein (PTHrP) in Plasma by LC-MS/MS. Methods Mol Biol. 2022;2546:375-389. doi:10.1007/978-1-0716-2565-1_33
IP
Publication Number: US-2016-0327577-A1
Patent Title: High Sensitivity Measurement of Parathyroid Hormone Related
Jurisdiction/Country: United States
Application Type: Non-Provisional
Contact Info
Jason Young
(801) 587-0519
jason.r.young@utah.edu
