Scaffold-free Chondrogenic Cell Sheets For Cartilage Regeneration
ID U-6819
Category Therapeutics
Subcategory Regenerative Medicine
Researchers
Brief Summary
A novel method for creating scaffold-free chondrogenic cell sheets from mesenchymal stem cells (MSCs) offers a direct transplantation solution for articular cartilage defects.
Problem Statement
Current cell therapies for cartilage regeneration fail to maintain hyaline-like phenotypes and strong adhesion to the cartilage surface, limiting their effectiveness.
Technology Description
This technology utilizes temperature-responsive cultureware to grow MSCs into confluent layers, which are then induced to differentiate into chondrogenic cells forming cell sheets. These sheets contract naturally to form a 3D structure that mimics hyaline cartilage, maintaining essential chondrogenic and structural characteristics. The method allows for direct transplantation of these cell sheets to the target tissue site, providing a viable alternative to current therapies that struggle with phenotype retention and tissue adherence.
Stage of Development
Pre-Clinical Validation
Benefit
- Hyaline-like Phenotype Retention: Maintains essential chondrogenic characteristics during in vitro culture.
- Strong Tissue Adhesion: Exhibits physical adhesion to cartilage surfaces post-transplantation, enhancing healing potential.
- Scaffold-Free Approach: Eliminates the need for scaffolds or cell injections, simplifying the transplantation process.
- Direct Transplantation Capability: Allows for the cell sheets to be moved directly from culture to the defect site, preserving cell integrity.
Publications
Thorp, Hallie et al. “Fabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets.” Scientific reports vol. 10,1 20869. 30 Nov. 2020
Thorp, Hallie et al. “Trends in Articular Cartilage Tissue Engineering: 3D Mesenchymal Stem Cell Sheets as Candidates for Engineered Hyaline-Like Cartilage.” Cells vol. 10,3 643. 13 Mar. 2021.
Thorp, Hallie et al. “Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering.” Regenerative therapy vol. 18 487-496. 2 Dec. 2021
CSTEC https://www.cstecutah.com/
Contact Info
Huy Tran
(801) 581-7792
huy.tran@utah.edu