Cyclic Peptide Libraries
ID U-6013
Category Research Tools (Tangible Property)
Subcategory Screening Assays
Researchers
Brief Summary
Cyclic peptide synthesis and production methods to create cyanobactin, divimides, and other cyclic peptide libraries for use in drug design, discovery, and therapeutics.
Problem Statement
Cyclic peptide libraries of novel compounds serve as valuable collections for researchers to mine for new therapeutics.
Technology Description
The proposed methods for creating cyclic peptide libraries utilize biosynthetic pathways from various organisms. The enzymatic steps are broken into individual components and then reassembled in unique ways to generate large collections of novel chemical entities. One method uses amide-linked peptides created in vivo by expression of constructs in E. coli. Another method centers on prenylated compounds created from marine ascidian enzymes, again expressed in E. coli. The final method utilizes natural pathways from sponges to synthesize compounds for libraries. Methods to increase the yield and potency of peptides, specifically cyanobactin and prenylated peptides, in the libraries have also been developed.
Stage of Development
Proof of Concept
Benefit
- Facilitates production of cyclic peptides libraries with unique novel chemical entities.
- Enables in vivo and in vitro production.
- Demonstrates potential for anticancer, antifungal, drug discovery, drug design, and drug screening applications.
- Expands scope to include proteins and non-peptide materials.
Publications
Zhang S, Qiu Y, Kakule T, et al (2017). Identification of cyclic depsipeptides and their dedicated synthetase from Hapsidospora irregularis. Journal of Natural Products. 80(2):363-370. doi: 10.1021/acs.jnatprod.6b00808
Otaka A, Aihara K, Inokuma T, et al. (2017). Cysteine-Free intramolecular ligation of N-Sulfanylethylanilide peptide using 4-Mercaptobenzylphosphonic Acid: Synthesis of cyclic peptide trichamide. Synlett. 28(15):1944-1949. doi: 10.1055/s-0036-1589055
IP
Publication Number: 2021/0040529 A1
Patent Title: METHODS FOR PRODUCING RIBOSOMALLY SYNTHESIZED AND POSTTRANSLATIONALLY MODIFIED PEPTIDES
Jurisdiction/Country: United States
Application Type: Non-Provisional
Contact Info
Jason Young
(801) 587-0519
jason.r.young@utah.edu